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Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

《化学科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 238-244 doi: 10.1007/s11705-016-1566-2

摘要： MATTt (a thermostable methionine adenosyltransferase from HB27) was overexpressed in and purified using Ni-NTA affinity column. The enzymatic activity of MATTt was investigated in a temperature range from 30 °C to 90 °C, showing that MATTt exhibited a high enzymatic activity and good thermostability at 80 °C. Circular dichroism spectra reveals that MATTt contains high portion of -sheet structures contributing to the thermostability of MATTt. The kinetic parameter, is 4.19 mmol/L and 1.2 mmol/L for ATP and methionine, respectively. MATTt exhibits the highest enzymatic activity at pH 8. Cobalt (Co ) and zinc ion (Zn ) enhances remarkably the activity of MATTt compared to the magnesium ion (Mg ). All these results indicated that the thermostable MATTt has great potential for industry applications.

关键词： ion-preference     methionine adenosyltransferase     secondary structure     thermostability     Thermus thermophilus    

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Mechanisms for simultaneous ozonation of sulfamethoxazole and natural organic matters in secondary effluent

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1368-0

摘要：

• SMX was mainly degraded by hydrolysis, isoxazole oxidation and double-bond addition.

关键词： Sulfamethoxazole     Ozonation     Natural organic matters     Secondary effluent     Degradation mechanism    

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Physical and chemical processes of wintertime secondary nitrate aerosol formation

Qi YING

《环境科学与工程前沿（英文）》 2011年 第5卷 第3期   页码 348-361 doi: 10.1007/s11783-011-0343-1

摘要： The UCD/CIT model was modified to include a process analysis (PA) scheme for gas and particulate matter (PM) to study the formation of secondary nitrate aerosol during a stagnant wintertime air pollution episode during the California Regional PM /PM Air Quality Study (CRPAQS) where detailed measurements of PM components are available at a few sites. Secondary nitrate is formed in the urban areas from near the ground to a few hundred meters above the surface during the day with a maximum modeled net increase rate of 4 μg·m ·d during the study episode. The secondary nitrate formation rate in rural areas is lower due to lower NO . In the afternoon hours, near-surface temperature can be high enough to evaporate the particulate nitrate. In the nighttime hours, both the gas phase N O reactions with water vapor and the N O heterogeneous reactions with particle-bound water are important for secondary nitrate formation. The N O reactions are most import near the surface to a few hundred meters above surface with a maximum modeled net secondary nitrate increase rate of 1 μg·m ·d and are more significant in the rural areas where the O concentrations are high at night. In general, vertical transport during the day moves the nitrate formed near the surface to higher elevations. During the stagnant days, process analysis indicates that the nitrate concentration in the upper air builds up and leads to a net downward flux of nitrate through vertical diffusion and a rapid increase of surface nitrate concentration.

关键词： secondary nitrate aerosol     N2O5 heterogeneous reaction     process analysis    

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Two-echelon Emergency Response Problem and Simulation Considering Secondary Disasters

Han-peng Zhang,Yi Liao,Hui-xia Luo

《工程管理前沿（英文）》 2014年 第1卷 第3期   页码 318-321 doi: 10.15302/J-FEM-2014042

摘要： It is necessary for subsequent resource distribution planning that get expected relief time considering secondary disasters after natural disaster. The goal of this research is to develop a two-echelon emergency resource distribution model under condition of secondary disasters. Taking minimal maximize relief time as criterion of relief performance, we developed response strategies and simulation model to get the expected value. Numerical studies of this paper presented the result of response strategies.

关键词： simulation     secondary disasters     two-echelon vehicle routing model    

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Formation of secondary inorganic aerosol in a frigid urban atmosphere

《环境科学与工程前沿（英文）》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1452-0

摘要：

•Harbin showed relatively high threshold RH (80%) for apparent increase of SOR.

关键词： Haze     Sulfate     Nitrate     Heterogeneous chemistry     Biomass burning     Northeast China    

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PCDD/Fs emission, risk characterization, and reduction in China’s secondary copper production industry

Haiqian LI, Yonglong LU, Li LI

《环境科学与工程前沿（英文）》 2013年 第7卷 第4期   页码 589-597 doi: 10.1007/s11783-013-0495-2

摘要： Secondary copper production is one of the key polychlorinated dibenzo- -dioxins and dibenzofurans (PCDD/Fs) emission sources in China, but research and data on this issue are rare. In 2004, when the Stockholm Convention entered into force in China, PCDD/Fs emissions from secondary copper production contributed to 32.2% of the total release. In this paper, PCDD/Fs emission dynamics from secondary copper industry were discussed and cumulative risks were characterized. From 2004 to 2009, industrial policies played an indirect role in PCDD/Fs reduction, but its effects are still limited. The Yangtze River Delta, Pearl River Delta and central regions were among the top three of dioxin emissions from secondary copper production in China. Shanghai, Shandong, Zhejiang, and Jiangxi had comparatively higher accumulated risk and were recommended as the priority regions for promoting PCDD/Fs emission control in China. From 2009 to 2015, the PCDD/Fs emission dynamics in the secondary copper industry were presented through simulation. PCDD/Fs emission equations were established, resulting in the recommendation of control technology conversion rate at 30% for small scale smelters and 51%–57% for large and medium-sized enterprises in 2015. In conclusion, both indirect policy and direct control technology retrofitting should be integrated for more effective PCDD/Fs emission reduction in secondary copper industry.

关键词： polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs)     secondary copper production     emission     risk     China    

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Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua

null

《医学前沿（英文）》 2017年 第11卷 第1期   页码 137-146 doi: 10.1007/s11684-016-0486-3

摘要：

This study aims to elucidate the underlying molecular mechanisms of artemisinin accumulation induced by cadmium (Cd). The effects of different Cd concentrations (0, 20, 60, and 120 μmol/L) on the biosynthesis of Artemisia annua L. were examined. Intermediate and end products were quantified by HPLC-ESI-MS/MS analysis. The expression of key biosynthesis enzymes was also determined by qRT-PCR. The results showed that the application of treatment with 60 and 120 μmol/L Cd for 3 days significantly improved the biosynthesis of artemisinic acid, arteannuin B, and artemisinin. The concentrations of artemisinic acid, arteannuin B, and artemisinin in the 120 μmol/L Cd-treated group were 2.26, 102.08, and 33.63 times higher than those in the control group, respectively. The concentrations of arteannuin B and artemisinin in 60 μmol/L Cd-treated leaves were 61.10 and 26.40 times higher than those in the control group, respectively. The relative expression levels of HMGR, FPS, ADS, CYP71AV1, DBR2, ALDH1, and DXR were up-regulated in the 120 μmol/L Cd-treated group because of increased contents of artemisinic metabolites after 3 days of treatment. Hence, appropriate doses of Cd can increase the concentrations of artemisinic metabolites at a certain time point by up-regulating the relative expression levels of key enzyme genes involved in artemisinin biosynthesis.

关键词： Cd     secondary metabolites     gene expressions     Artemisia annua L.    

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Simple model of sludge thickening process in secondary settlers

Yuankai ZHANG,Hongchen WANG,Lu QI,Guohua LIU,Zhijiang HE,Songzhu JIANG

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 319-326 doi: 10.1007/s11783-014-0758-6

摘要： In wastewater treatment plants (WWTPs), a secondary settler acts as a clarifier, sludge thickener, and sludge storage tank during peak flows and therefore plays an important role in the performance of the activated sludge process. Sludge thickening occurs in the lower portions of secondary clarifiers during their operation. In this study, by detecting the hindered zone from the complete thickening process of activated sludge, a simple model for the sludge thickening velocity, , describing the potential and performance of activated sludge thickening in the hindered zone was developed. However, sludge thickening in the compression zone was not studied because sludge in the compression zone showed limited thickening. This empirical model was developed using batch settling data obtained from four WWTPs and validated using measured data from a fifth WWTP to better study sludge thickening. To explore different sludge settling and thickening mechanisms, the curves of sludge thickening and sludge settling were compared. Finally, it was found that several factors including temperature, stirring, initial depth, and polymer conditioning can lead to highly concentrated return sludge and biomass in a biologic reactor.

关键词： wastewater treatment plants     secondary settler     sludge thickening     sludge settling     hindered zone    

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On the location of the secondary instantaneous poles in two-degree-of-freedom spherical mechanisms

Soheil ZARKANDI

《机械工程前沿（英文）》 2014年 第9卷 第1期   页码 34-40 doi: 10.1007/s11465-014-0290-1

摘要：

As the instant centers in planar mechanisms, the instantaneous poles (or instant poles, in brief) can be used for kinematic analysis in spherical mechanisms. One of the mandatory steps in this analysis is the determination of the location of these poles. This paper presents a theorem showing analytically that the locus of an unknown secondary instant pole in two-degree-of-freedom (2-DOF) spherical mechanisms is a great circle (GC). The exact location of the pole on its GC is obtained based on the configuration of the mechanism and velocity ratio of the two inputs. Moreover, using the results of the theorem, a geometrical technique is presented to determine the GC of the pole.

关键词： spherical mechanisms     instantaneous poles     great circle     angular velocity    

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Optical performance analysis of an innovative linear focus secondary trough solar concentrating system

Xiliang ZHANG, Zhiying CUI, Jianhan ZHANG, Fengwu BAI, Zhifeng WANG

《能源前沿（英文）》 2019年 第13卷 第3期   页码 590-596 doi: 10.1007/s11708-018-0602-y

摘要： The parabolic trough solar concentrating system has been well developed and widely used in commercial solar thermal power plants. However, the conventional system has its drawbacks when connecting receiver tube parts and enhancing the concentration ratio. To overcome those inherent disadvantages, in this paper, an innovative concept of linear focus secondary trough concentrating system was proposed, which consists of a fixed parabolic trough concentrator, one or more heliostats, and a fixed tube receiver. The proposed system not only avoids the end loss and connection problem on the receiver during the tracking process but also opens up the possibility to increase the concentration ratio by enlarging aperture. The design scheme of the proposed system was elaborated in detail in this paper. Besides, the optical performance of the semi and the whole secondary solar trough concentrator was evaluated by using the ray tracing method. This innovative solar concentrating system shows a high application value as a solar energy experimental device.

关键词： secondary parabolic trough solar concentrator     ray tracing method     linear focus     concentration ratio     optical performance    

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Relationship between the number of tapping-induced secondary laticifer lines and rubber yield among

Yueyi CHEN,Xinsheng GAO,Xiaofei ZHANG,Weimin TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第4期   页码 363-367 doi: 10.15302/J-FASE-2016122

摘要： The lack of suitable early selection parameters means that traditional rubber breeding for yield is time-consuming and inefficient. Laticifer is a tissue specific for natural rubber biosynthesis and storage in rubber tree. The number of the secondary laticifers in the trunk bark tissues is positively correlated with rubber yield in the matured rubber trees that are regularly tapped. In the present study, the rubber yield from 280 of 4–5 year-old virgin trees from 7 cross combinations was compared with the number of newly differentiated secondary laticifers caused by tapping. Results showed that the number of tapping-induced lines of secondary laticifers varied in different germplasm and was positively related to the rubber yield, indicating this could be used as a suitable parameter for early evaluation of yield potential of rubber trees.

关键词： Hevea brasiliensis     rubber yield breeding     early evaluation     tapping     secondary laticifer differentiation    

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知识发现领域中当今面临的五类重大问题

杨炳儒

《中国工程科学》 2009年 第11卷 第4期   页码 76-83

摘要：

系统地总结与提出知识发现(KD)领域中当今面临的五类重大问题，它们是KD中的两大核心问题、两大猜想问题、主流发展中富有挑战性的问题、应用研究中的相关领域重大问题以及KD技术标准的制定问题，并给出其部分成果或具体分析。这五类问题密切相关，对它们的研究必将KD推向新的发展阶段,并在该领域内外产生深刻的影响。

关键词： KD核心问题     KD猜想     KD挑战性问题     蛋白质二级结构预测     KD技术标准    

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A method for quantifying bias in modeled concentrations and source impacts for secondary particulate

Cesunica E. Ivey, Heather A. Holmes, Yongtao Hu, James A. Mulholland, Armistead G. Russell

《环境科学与工程前沿（英文）》 2016年 第10卷 第5期 doi: 10.1007/s11783-016-0866-6

摘要： A method for quantifying source impacts for secondary PM species is derived. The method provides estimates of bias in modeled concentrations. Adjusted concentrations match corresponding observations at monitored locations. Sources impacts on secondary species are estimated over the US for 20 sources. Community Multi-Scale Air Quality (CMAQ) estimates of sulfates, nitrates, ammonium, and organic carbon are highly influenced by uncertainties in modeled secondary formation processes, such as chemical mechanisms, volatilization, and condensation rates. These compounds constitute the majority of PM mass, and reducing bias in estimated concentrations has benefits for policy measures and epidemiological studies. In this work, a method for adjusting source impacts on secondary species is developed that provides estimates of source contributions and reduces bias in modeled concentrations compared to observations. The bias correction adjusts concentrations and source impacts based on the difference between modeled concentrations and observations while taking into account uncertainties at the location of interest; and it is applied both spatially and temporally. We apply the method over the US for 2006. The mean bias for initial CMAQ concentrations compared to observations is −0.28 (OC), 0.11 (NO ), 0.05 (NH ), and −0.08 (SO ). The normalized mean bias in modeled concentrations compared to observations was effectively zero for OC, NO , NH , and SO after applying the secondary bias correction. 10-fold cross-validation was conducted to determine the performance of the spatial application of the bias correction. Cross-validation performance was favorable; correlation coefficients were greater than 0.69 for all species when comparing observations and concentrations based on kriged correction factors. The methods presented here address model uncertainties by improving simulated concentrations and source impacts of secondary particulate matter through data assimilation. Secondary-adjusted concentrations and source impacts from 20 emissions sources are generated for 2006 over continental US.

关键词： Particulate matter     Source apportionment     Secondary particulate matter     Chemical transport modeling     Receptor modeling    

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Effects of two transition metal sulfate salts on secondary organic aerosol formation in toluene/NO

Biwu CHU, Jiming HAO, Junhua LI, Hideto TAKEKAWA, Kun WANG, Jingkun JIANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 1-9 doi: 10.1007/s11783-012-0476-x

摘要： Aerosol phase reactions play a very important role on secondary organic aerosol (SOA) formation, and metal-containing aerosols are important components in the atmosphere. In this study, we tested the effects of two transition metal sulfate salts, manganese sulfate (MnSO ) and zinc sulfate (ZnSO ), on the photochemical reactions of a toluene/NO photooxidation system in a 2 m smog chamber. By comparing photochemical reaction products of experiments with and without transition metal sulfate seed aerosols, we evaluated the effects of transition metal sulfate seed aerosols on toluene consumption, NO conversion and the formation of ozone and SOA. MnSO and ZnSO seed aerosols were found to have similar effects on photochemical reactions, both enhance the SOA production, while showing negligible effects on the gas phase compounds. These observations are consistent when varying metal sulfate aerosol concentrations. This is attributed to the catalytic effects of MnSO and ZnSO seed aerosols which may enhance the formation of condensable semivolatile compounds. Their subsequent partitioning into the aerosol phase leads to the observed SOA formation enhancement.

关键词： manganese sulfate     zinc sulfate     seed aerosols     toluene photooxidation     secondary organic aerosol    

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DNA组装效率的无偏差快速检验——基于qPCR而不依赖于转化的方法 Article

马晓焉, 梁昕鑫, 霍毅欣

《工程（英文）》 2019年 第5卷 第4期   页码 803-810 doi: 10.1016/j.eng.2019.06.002

摘要：

合成生物学正朝着大规模、复杂化的方向发展，这在很大程度上依赖于基因模块的高效组装。传统评估DNA组装效率（AE）的方法需要进行转化，整个过程耗时长达10 h，而且容易受到各种因素的干扰。为快速、可靠地测定组装效率，本研究建立了一种基于qPCR的不依赖于转化的测定方法，用连接上的片段占初始加入片段的比例表征组装效率，3 h即可完成测定。利用该方法测定了酶切连接、Golden Gate组装以及Gibson组装法的双片段或多片段组装效率，所得结果与菌落计数法表征的组装效率呈显著正相关。该方法消除了转化过程的随机性，降低了测定偏差，优于传统的菌落计数法。随后，用此方法研究了DNA片段末端的二级结构对组装效率的影响。结果显示，所有依赖于末端序列互补的组装技术，其组装效率主要受重叠序列整体性质的影响，而发夹结构可显著降低组装效率。这种基于qPCR的测定方法将促进DNA组装技术的发展，并有助于对组装效率影响因素的评估。

关键词： 组装效率     DNA组装     qPCR     二级结构     转化    

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标题 作者 时间 类型 操作

Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

期刊论文

Mechanisms for simultaneous ozonation of sulfamethoxazole and natural organic matters in secondary effluent

期刊论文

Physical and chemical processes of wintertime secondary nitrate aerosol formation

Qi YING

期刊论文

Two-echelon Emergency Response Problem and Simulation Considering Secondary Disasters

Han-peng Zhang,Yi Liao,Hui-xia Luo

期刊论文

Formation of secondary inorganic aerosol in a frigid urban atmosphere

期刊论文

PCDD/Fs emission, risk characterization, and reduction in China’s secondary copper production industry

Haiqian LI, Yonglong LU, Li LI

期刊论文

Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua

null

期刊论文

Simple model of sludge thickening process in secondary settlers

Yuankai ZHANG,Hongchen WANG,Lu QI,Guohua LIU,Zhijiang HE,Songzhu JIANG

期刊论文

On the location of the secondary instantaneous poles in two-degree-of-freedom spherical mechanisms

Soheil ZARKANDI

期刊论文

Optical performance analysis of an innovative linear focus secondary trough solar concentrating system

Xiliang ZHANG, Zhiying CUI, Jianhan ZHANG, Fengwu BAI, Zhifeng WANG

期刊论文

Relationship between the number of tapping-induced secondary laticifer lines and rubber yield among

Yueyi CHEN,Xinsheng GAO,Xiaofei ZHANG,Weimin TIAN

期刊论文

知识发现领域中当今面临的五类重大问题

杨炳儒

期刊论文

A method for quantifying bias in modeled concentrations and source impacts for secondary particulate

Cesunica E. Ivey, Heather A. Holmes, Yongtao Hu, James A. Mulholland, Armistead G. Russell

期刊论文

Effects of two transition metal sulfate salts on secondary organic aerosol formation in toluene/NO

Biwu CHU, Jiming HAO, Junhua LI, Hideto TAKEKAWA, Kun WANG, Jingkun JIANG

期刊论文

DNA组装效率的无偏差快速检验——基于qPCR而不依赖于转化的方法

马晓焉, 梁昕鑫, 霍毅欣

期刊论文